Impact of Chlorhexidine Digluconate on Bacterial DNA Preservation and PCR Efficiency
D. L. Zornikov,
D. M. Nechaeva,
V. M. Simarzina,
D. O. Kornilov,
A. E. Kariakina,
P. G. Amineva,
E. S. Voroshilina https://doi.org/10.69964/BMCC-2025-2-1-79-85
Abstract
Introduction. A 0.05% solution of chlorhexidine digluconate (CD) is one of the most commonly used antiseptics, however it is unclear whether CD has an inhibitory effect on nucleicacid amplification during polymerase chain reaction (PCR). The aim of the study. To evaluate the impact of chlorhexidine digluconate on bacterial DNA preservation and PCR efficiency. Materials and methods. The susceptibility of Lactobacillus acidophilus, Staphylococcus aureus, and Escherichia coli mixture to 0.05% CD solution was studied after 1, 2, and 7-day of exposure in a refrigerator and freezer. Results. The CD solution did not cause PCR inhibition or destruction of bacterial DNA of any tested bacterial groups after 1, 2, or 7 days of exposure under the refrigerator and freezer conditions. Conclusions. The results obtained allow to interpret PCR tests for bacterial infections and the microbiota composition in a regular way, even after pretreatment of the mucosa with a 0.05% chlorhexidine digluconate solution.
About the Authors
D. L. Zornikov
Ural State Medical University
Russian Federation
Russian Federation
Danila L. Zornikov — Candidate of Sciences (Medicine), Associate Professor, Associate Professor of the Department of Medical Microbiology and Clinical Laboratory Diagnostics
Address: st. Repina, 3, Ekaterinburg, 620028
D. M. Nechaeva
Ural State Medical University
Russian Federation
Russian Federation
Diana M. Nechaeva — Laboratory Technician of the Department of Medical Microbiology and Clinical Laboratory Diagnostics
Address: st. Repina, 3, Ekaterinburg, 620028
V. M. Simarzina
Ural State Medical University
Russian Federation
Russian Federation
Veronika M. Simarzina — Assistant Professor of the Department of Medical Microbiology and Clinical Laboratory Diagnostics
Address: st. Repina, 3, Ekaterinburg, 620028
D. O. Kornilov
Ural State Medical University
Russian Federation
Russian Federation
Daniil O. Kornilov — Assistant Professor of the Department of Medical Microbiology and Clinical Laboratory Diagnostics
Address: st. Repina, 3, Ekaterinburg, 620028
A. E. Kariakina
Ural State Medical University
Russian Federation
Russian Federation
Anastasia E. Kariakina — Laboratory Technician of the Department of Medical Microbiology and Clinical Laboratory Diagnostics
Address: st. Repina, 3, Ekaterinburg, 620028
P. G. Amineva
Ural State Medical University; LLC “Quality Med”
Russian Federation
Russian Federation
Polina G. Amineva — Assistant Professor of the Department of Medical Microbiology and Clinical Laboratory Diagnostics; bacteriologist, Head of Laboratory
Address: st. Mashinnaya, 1, Ekaterinburg, 620028
E. S. Voroshilina
Ural State Medical University; “Garmonia” Medical Center
Russian Federation
Russian Federation
Ekaterina S. Voroshilina — Doctor of Sciences (Medicine), Professor, Head of the Department of Medical Microbiology and Clinical Laboratory Diagnostics; Head of Laboratories
Address: st. Tveritina, 16, Ekaterinburg, 620028
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Review
For citations:
Zornikov D.L., Nechaeva D.M., Simarzina V.M., Kornilov D.O., Kariakina A.E., Amineva P.G., Voroshilina E.S. Impact of Chlorhexidine Digluconate on Bacterial DNA Preservation and PCR Efficiency. Bulletin of maternal and child care. 2025;2(1):79-85. (In Russ.) https://doi.org/10.69964/BMCC-2025-2-1-79-85
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