Genetic profile of antibiotic resistance of enterobacteria strains isolated from obstetrics, gynecology and pediatric patients

Background. Antibiotic resistance of microorganisms is a significant problem for public health in countries around the world. Studying the structure and prevalence of genetic determinants of antibiotic resistance is a current research area that makes it possible to determine the mechanisms of resistance to antibacterial drugs.

Purpose of the study. To study the genetic profile of antibiotic resistance of Enterobacteriaceae strains isolated from obstetrics, gynecology and pediatric patients.

Materials and methods. The genetic profile of antibiotic resistance was determined in ESBL-producing strains of Enterobacteriaceae isolated from samples of biological material obtained from 43 women and 24 children hospitalized in the departments of the Federal State Budgetary Institution “Research Institute of OMM” of the Russian Ministry of Health in 2024. The DNA of bacterial cells of ESBL-producing isolates was isolated from a daily culture of microorganisms using the PROBA-NK kit. Detection of genes blatem, blactx-M-1, blashv; blaoxa-40-like, blaoxa-48-like, blaоxa-23-like, blaoxa-51-like, blaimp, blaKPS, blaNDM, were carried out using the BakResist GLA kit on a DT-48 detection amplifier (DNA technology, Russia ).

Results. In representatives of the Enterobacteriaceae family isolated from children, the blactx-M-1+blashv genovariant was identified in 29.2% of cases and was found only in Klebsiella pneumoniae. Blatem+blactx-M-1 was detected in 20.8%, and blactx-M-1+blashv+blatem  — in 12%. Among representatives of the Enterobacteriaceae family isolated from women, the blactx-M-1 genovariant was found in 37.2% of cases and was detected only in Escherichia coli. And the options blactx-M-1+blatem and blactx-M-1+blashv — in 16.3%. In 2024, for the first time in 7 years of our research to identify the genetic determinants of antibiotic resistance, a multidrug-resistant Escherichia coli strain with the blaNDM gene was identified. Also noteworthy is the identification of K. pneumoniae with the genetic profile of antibiotic resistance blatem, blactx-M-1, blashv, blaoxa-48-like, blaNDM.

Conclusion. The genetic profile of antibiotic resistance of Enterobacteriaceae strains isolated from patients in obstetrics, gynecology and pediatric departments is represented by 8 genovariants, in which the dominant gene providing resistance to beta-lactam antibiotics among Enterobacteriaceae in 2024, as in 2021, 2022, blaCTX-M-1 remains, which is found both as a monovariant and in association with blatem and blashv.

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